This is two coats followed by BECCA top coat. Unfortunately, it must not have been completely dry before I went to bed as there are imprints which spoil the finish somewhat! The polish went on easily. I had to be careful to wipe the brush off before applying though, as if the brush was overloaded, I found the polish could go on a little streaky. I found the brush easy to use but I do wish the handle was a little bigger.
As ever, I cannot offer any comments on the wear. I believe Illamasqua polishes are generally pretty good in terms of wear time. I have nothing similar in my collection to compare this colour to but I spotted a very interesting comparisons of Bacterium to Chanel Quartz from the Illusions d'Ombre de Chanel on The Swatchaholic. They're not dupes but they're quite similar when applied. This is certainly a useful comparison for anyone pondering the Chanel Autumn collection.